References- Part 2 of paper (part 1)
Is a Positive Western Blot Proof of HIV Infection?
Bio/Technology Vol.11 June 1993
Eleni Papadopulos-Eleopulos, Valendar F. Turner and John M. Papadimitriou

Acknowledgements

We wish to thank all our colleagues and especially Udo SchEklenk, Barry Page, Bruce Hedland-Thomas, David Causer, Richard Fox, John Peacock, David Prentice, Ronald Hirsch, Patricia Shalala, Keith Jones, Alun Dufty, June Rider Jones, Coronary Barrow, Dorothy Davis, Julian Smith, Mark Strahan, Vincent Turner, Wallace Turner and Graham Drabble for their continued support and assistance.

Dedication

This work is dedicated to the memory of Methodios Papadopulos and Margaret Joan Turner.

Authors:

Eleni Papadopulos-Eleopulos, Physicist
Department of Medical Physics
Royal Perth Hospital

Valendar F. Turner, Staff Specialist
Department of Emergency Medicine
Royal Perth Hospital

John M. Papadimitriou Professor of Pathology
Department of Pathology
University of Western Australia

Correspondence to:

Eleni Papadopulos-Eleopulos
Department of Medical Physics
Royal Perth Hospital
Box X2213 GPO Perth

Captions for figures 0-4.

Figure 0.(left out with publication)

WB patterns with patient sera "and reaction with a strong, weak and non-reactive control". (Reproduced from Bio-Rad Laboratory Manual).

Figure 1.

(A): "Cord blood T-lymphocytes infected with virus" (HIV-1) were lysed and the supernatant of a 10,000g centrifugation of the cell lysate was immunoprecpitated with sera from patients with lymphadenopathy (P); a healthy donor (h); goat antiserum to HTLV-I p24 (G); normal goat serum (g).

(B): As 1A but cells infected with HTLV-I instead of HIV-1. 2C: The cell free supernatant from the cultures of "cord blood T-lymphocytes infected with virus" (HIV-1) was ultracentrifuged for one hour at 50,000 rev/min.The pellett was banded in sucrose density gradients. Material which banded at 1.16gm/ml (the complete virus) was immunoprecipitated with the above sera but instead of normal goat serum, serum from another healthy donor (h) was used. Although in the published strips it is hard if not impossible to distinguish any bands, in the text, it is stated that "three major proteins could be seen: the p25 protein and proteins with molecular weights of 80,000 and 45,000" (Modifed from BarrG-Sinoussi et al. Science Vol 220:p870).

Figure 2.

(A): "Lysates of HTLV-III producer" H4 clone cells, derived from the HUT78 cell line immunoprecipitated with various sera.

(B): "Lysates of HTLV-III producer" H17 clone cells also derived from the HUT78 cell line, immunoprecipitated with various sera; (the serum in B lane 2 is identical to (A) Lane 4).

(C): Lysates of H17 and H4 clones (b) "before" and (a) "after infection", immunoprecipitated with serum from a male heterosexual drug user with lymphandenopathy and thrombocytopenia (pre-AIDS). This is the same serum as (B) Lane 5.

(D): "Lysates of H4/HTLV-III... cells" (C), or "virus purified from the cells culture fluids", (V), using (I)-same serum as (B) Lane 5; (II)-serum from a patient with pre-AIDS; (III) serum from a patient with AIDS. This is the same serum as (B) Lane 4.

Key to sera: (A) AIDS patient; (P) pre-AIDS patient; (h) healthy control; (U) drug user; (H) homosexual control; (Modified from Schubach et al 1984. Science Vol 224:p504).

Figure 3.

WB of one and the same serum specimen tested by 19 laboratories. (From Lundberg GD 1988. JAMA Vol 260:p676).

Figure 4.

Structural model of HIV. From reference 107.

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